Cell viability assay protocol for evaluating the biocompatibility of nanomaterials using the MTT colorimetric method.
Biological Protocol
This protocol describes the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay for assessing nanoparticle cytotoxicity in cultured cells. The method quantifies cell viability based on mitochondrial metabolic activity and provides IC₅₀ values for nanoparticle formulations. The protocol includes cell seeding, nanoparticle treatment, MTT incubation, and colorimetric measurement.
Medic Tech. (2026). MTT Assay for Nanoparticle Cytotoxicity Assessment. Protocol ID: proto-261-o48v. Retrieved from https://protoly.net/proto-261-o48v
Medic Tech. "MTT Assay for Nanoparticle Cytotoxicity Assessment." Protocol ID proto-261-o48v, 2026. Web. 12 Apr 2026.
Medic Tech. "MTT Assay for Nanoparticle Cytotoxicity Assessment." Protocol ID: proto-261-o48v. Accessed April 12, 2026. https://protoly.net/proto-261-o48v.